Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The E3 ubiquitin ligase CHIP mediates ubiquitination and proteasomal degradation of PRMT5.

Zhang HT, Zeng LF, He QY, Tao WA, Zha ZG, Hu CD

Protein arginine methyltransferase 5 (PRMT5) is an important member of the protein arginine methyltransferase family that regulates many cellular processes through epigenetic control of target gene expression. Because of its overexpression in a number of human cancers and its essential role in cell proliferation, transformation, and cell cycle progression, PRMT5 has been recently proposed to function as an oncoprotein in ... [more]

Biochim. Biophys. Acta Feb. 01, 2016; 1863(2);335-46 [Pubmed: 26658161]

Throughput

  • Low Throughput

Additional Notes

  • Table 1

Curated By

  • BioGRID