ARFGAP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CLTC
Gene Ontology Biological Process
- Golgi organization [IMP]
- mitotic nuclear division [IMP, ISO]
- negative regulation of hyaluronan biosynthetic process [ISO, ISS]
- negative regulation of protein localization to plasma membrane [ISO]
- osteoblast differentiation [ISO]
- receptor internalization [ISO]
- receptor-mediated endocytosis [IMP, ISO]
- transferrin transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [IDA]
- clathrin coat [IDA, TAS]
- clathrin complex [ISO]
- clathrin-coated endocytic vesicle membrane [TAS]
- clathrin-coated vesicle [ISO]
- cytoplasm [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- intracellular membrane-bounded organelle [ISO]
- membrane [ISO]
- membrane coat [ISO]
- mitochondrion [ISO]
- myelin sheath [ISO]
- protein complex [ISO]
- sarcolemma [IDA]
- spindle [IDA, ISO]
- terminal bouton [IDA]
- vesicle [ISO]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
ARFGAP1 promotes AP-2-dependent endocytosis.
COPI (coat protein I) and the clathrin-AP-2 (adaptor protein 2) complex are well-characterized coat proteins, but a component that is common to these two coats has not been identified. The GTPase-activating protein (GAP) for ADP-ribosylation factor 1 (ARF1), ARFGAP1, is a known component of the COPI complex. Here, we show that distinct regions of ARFGAP1 interact with AP-2 and coatomer ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ARFGAP1 CLTC | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID