BAIT

HOXA2-A

X-Hoxa2, hox1k, hoxa2, hoxa2b, hoxa2b-A
homeobox A2
GO Process (0)
GO Function (0)
GO Component (0)
Xenopus laevis
PREY

RCHY1

ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase
GO Process (0)
GO Function (0)
GO Component (0)
Xenopus laevis

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Molecular Analysis of the HOXA2-Dependent Degradation of RCHY1.

Bridoux L, Deneyer N, Bergiers I, Rezsohazy R

The homeodomain transcription factor Hoxa2 interacts with the RING-finger type E3 ubiquitin ligase RCHY1 and induces its proteasomal degradation. In this work, we dissected this non-transcriptional activity of Hoxa2 at the molecular level. The Hoxa2-mediated decay of RCHY1 involves both the 19S and 20S proteasome complexes. It relies on both the Hoxa2 homeodomain and C-terminal moiety although no single deletion ... [more]

PLoS ONE Oct. 27, 2015; 10(10);e0141347 [Pubmed: 26496426]

Throughput

  • Low Throughput

Additional Notes

  • BiFC

Curated By

  • BioGRID