GCKR
Gene Ontology Biological Process
- cellular glucose homeostasis [IMP]
- negative regulation of catalytic activity [ISO]
- negative regulation of glucokinase activity [IMP, ISO]
- positive regulation of glucokinase activity [IMP]
- protein import into nucleus, translocation [IMP, ISO]
- protein localization to nucleus [IMP]
- response to fructose [ISO]
- triglyceride homeostasis [ISO]
- urate metabolic process [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell [IMP]
- cytoplasm [ISO]
- cytosol [ISO]
- intracellular [IMP]
- mitochondrion [ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
GCK
Gene Ontology Biological Process
- NADP metabolic process [IMP]
- calcium ion import [IMP]
- carbohydrate phosphorylation [IDA, IMP, ISO]
- cellular glucose homeostasis [IMP, ISO]
- cellular response to glucose starvation [ISO]
- cellular response to insulin stimulus [ISO]
- cellular response to leptin stimulus [ISO]
- detection of glucose [ISO]
- fructose 2,6-bisphosphate metabolic process [ISO]
- glucose 6-phosphate metabolic process [IMP, ISO]
- glucose homeostasis [IMP, ISO]
- glucose metabolic process [IDA, IMP, ISO]
- glycogen biosynthetic process [ISO]
- glycolytic process [IBA, ISO]
- lipid homeostasis [ISO]
- negative regulation of epinephrine secretion [ISO]
- negative regulation of gluconeogenesis [ISO]
- positive regulation of cytosolic calcium ion concentration [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of glycolytic process [ISO]
- positive regulation of insulin secretion [IMP, ISO]
- positive regulation of phosphorylation [ISO]
- regulation of insulin secretion [IMP, ISO]
- regulation of potassium ion transport [IMP]
- second-messenger-mediated signaling [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin filament [ISO]
- basal cortex [ISO]
- cell [IMP]
- cell cortex [ISO]
- cytoplasm [ISO]
- cytosol [IBA, IDA, ISO]
- mitochondrion [IDA, ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- secretory granule [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Acetylation of glucokinase regulatory protein decreases glucose metabolism by suppressing glucokinase activity.
Glucokinase (GK), mainly expressed in the liver and pancreatic β-cells, is critical for maintaining glucose homeostasis. GK expression and kinase activity, respectively, are both modulated at the transcriptional and post-translational levels. Post-translationally, GK is regulated by binding the glucokinase regulatory protein (GKRP), resulting in GK retention in the nucleus and its inability to participate in cytosolic glycolysis. Although hepatic GKRP ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 5
Curated By
- BioGRID