BAIT

EIF3F

0610037M02Rik, AA409853, Eif3s5
eukaryotic translation initiation factor 3, subunit F
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The eukaryotic translation initiation factor 3f (eIF3f) interacts physically with the alpha 1B-adrenergic receptor and stimulates adrenoceptor activity.

Gutierrez-Fernandez MJ, Higareda-Mendoza AE, Gomez-Correa CA, Pardo-Galvan MA

eIF3f is a multifunctional protein capable of interacting with proteins involved in different cellular processes, such as protein synthesis, DNA repair, and viral mRNA edition. In human cells, eIF3f is related to cell cycle and proliferation, and its deregulation compromises cell viability.We here report that, in native conditions, eIF3f physically interacts with the alpha 1B-adrenergic receptor, a plasma membrane protein ... [more]

BMC Biochem. Oct. 27, 2015; 16(0);25 [Pubmed: 26497985]

Throughput

  • Low Throughput

Additional Notes

  • Figure 2

Curated By

  • BioGRID