An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Regulation of Itch and Nedd4 E3 Ligase Activity and Degradation by LRAD3.

Noyes NC, Hampton B, Migliorini M, Strickland DK

Itch and Nedd4 are members of the Nedd4 family of E3 ubiquitin ligases that are important in a number of biological processes. Precise regulation of their enzymatic activity is required for normal physiological function. Nedd4-like E3 ligases exist in an inactive form resulting from intramolecular interactions of their catalytic HECT domain with their WW domains. We identified the low-density-lipoprotein receptor ... [more]

Biochemistry Mar. 01, 2016; 55(8);1204-13 [Pubmed: 26854353]

Throughput

Low Throughput

Additional Notes

Figure 1
identified by mass spec

Curated By

BioGRID

Interaction Summary

LDLRAD3

Gene Ontology Cellular Component

DNM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [ISO]nitric-oxide synthase binding [ISO]poly(A) RNA binding [ISO]protein C-terminus binding [ISO]protein binding [IPI]protein complex binding [ISO]protein dimerization activity [IDA]