BAIT
PARK2
Park, Prkn
parkin RBR E3 ubiquitin protein ligase
GO Process (63)
GO Function (22)
GO Component (22)
Gene Ontology Biological Process
- adult locomotory behavior [ISO, ISS]
- aggresome assembly [ISO, ISS]
- cellular protein catabolic process [ISO]
- cellular protein modification process [TAS]
- cellular response to toxic substance [ISO, ISS]
- dopamine metabolic process [ISO]
- dopamine uptake involved in synaptic transmission [ISO]
- learning [ISO]
- locomotory behavior [ISO]
- mitochondrial fission [ISS]
- mitochondrion degradation [ISO, ISS]
- mitochondrion organization [ISS]
- negative regulation of JNK cascade [ISS]
- negative regulation of actin filament bundle assembly [ISO]
- negative regulation of cell death [ISO]
- negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [ISO, ISS]
- negative regulation of glucokinase activity [ISO, ISS]
- negative regulation of insulin secretion [ISO, ISS]
- negative regulation of mitochondrial fusion [ISS]
- negative regulation of neuron apoptotic process [ISO, ISS]
- negative regulation of neuron death [IGI, ISO]
- negative regulation of oxidative stress-induced neuron death [IGI]
- negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [ISO, ISS]
- negative regulation of protein phosphorylation [ISO, ISS]
- negative regulation of reactive oxygen species metabolic process [ISO, ISS]
- negative regulation of release of cytochrome c from mitochondria [ISO, ISS]
- neuron cellular homeostasis [ISS]
- neuron maturation [IEP]
- norepinephrine metabolic process [ISO]
- positive regulation of DNA binding [ISO, ISS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO, ISS]
- positive regulation of mitochondrial fission [ISS]
- positive regulation of mitochondrial fusion [ISO, ISS]
- positive regulation of proteasomal protein catabolic process [ISO]
- positive regulation of protein linear polyubiquitination [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO, ISS]
- positive regulation of tumor necrosis factor-mediated signaling pathway [ISO, ISS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO, ISS]
- protein K48-linked ubiquitination [ISO, ISS]
- protein K63-linked ubiquitination [ISO, ISS]
- protein autoubiquitination [ISO, ISS]
- protein localization to mitochondrion [ISS]
- protein metabolic process [ISO]
- protein monoubiquitination [ISO, ISS]
- protein polyubiquitination [ISO, ISS]
- protein ubiquitination [ISO, ISS]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO, ISS]
- regulation of autophagy [ISO, ISS]
- regulation of cellular response to oxidative stress [ISS]
- regulation of mitochondrion organization [ISO, ISS]
- regulation of neurotransmitter secretion [ISO]
- regulation of reactive oxygen species metabolic process [ISO]
- regulation of synaptic transmission [ISO]
- response to corticosterone [IEP]
- response to drug [IEP]
- response to endoplasmic reticulum stress [ISO]
- response to oxidative stress [ISS]
- response to unfolded protein [IDA]
- startle response [ISO]
- synaptic transmission, dopaminergic [ISO]
- synaptic transmission, glutamatergic [ISO]
- ubiquitin-dependent protein catabolic process [TAS]
- zinc ion homeostasis [ISS]
Gene Ontology Molecular Function- F-box domain binding [ISO, ISS]
- G-protein coupled receptor binding [ISO, ISS]
- Hsp70 protein binding [ISO, ISS]
- PDZ domain binding [ISO, ISS]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO, ISS]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO, ISS]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [IDA]
- protein kinase binding [ISO, ISS]
- transcription regulatory region sequence-specific DNA binding [ISS]
- tubulin binding [ISO]
- ubiquitin binding [ISO, ISS]
- ubiquitin conjugating enzyme binding [ISO, ISS]
- ubiquitin protein ligase activity [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO, ISS, TAS]
- ubiquitin-specific protease binding [ISO]
- F-box domain binding [ISO, ISS]
- G-protein coupled receptor binding [ISO, ISS]
- Hsp70 protein binding [ISO, ISS]
- PDZ domain binding [ISO, ISS]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO, ISS]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO, ISS]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [IDA]
- protein kinase binding [ISO, ISS]
- transcription regulatory region sequence-specific DNA binding [ISS]
- tubulin binding [ISO]
- ubiquitin binding [ISO, ISS]
- ubiquitin conjugating enzyme binding [ISO, ISS]
- ubiquitin protein ligase activity [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO, ISS, TAS]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- Golgi membrane [IDA]
- LUBAC complex [ISO, ISS]
- Parkin-FBXW7-Cul1 ubiquitin ligase complex [ISO, ISS]
- SCF ubiquitin ligase complex [ISO]
- aggresome [ISO, ISS]
- cytoplasm [IDA, ISO, ISS]
- cytoplasmic vesicle [IDA]
- cytosol [ISO, ISS]
- endoplasmic reticulum [IDA, ISO]
- endoplasmic reticulum membrane [IDA]
- intracellular [IDA]
- membrane raft [IDA]
- mitochondrion [ISO, ISS]
- neuron projection [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- postsynaptic density [IDA]
- protein complex [ISO]
- synapse [IDA]
- synaptic vesicle [IDA]
- ubiquitin ligase complex [ISO, ISS]
Rattus norvegicus
PREY
UBC
HMG20
ubiquitin C
GO Process (75)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [TAS]
- DNA repair [TAS]
- Fc-epsilon receptor signaling pathway [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- I-kappaB kinase/NF-kappaB signaling [TAS]
- JNK cascade [TAS]
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- Notch receptor processing [TAS]
- Notch signaling pathway [TAS]
- RNA metabolic process [TAS]
- T cell receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- activation of MAPK activity [TAS]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- apoptotic process [TAS]
- apoptotic signaling pathway [TAS]
- carbohydrate metabolic process [TAS]
- cellular response to hypoxia [TAS]
- cytokine-mediated signaling pathway [TAS]
- endosomal transport [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- glucose metabolic process [TAS]
- glycogen biosynthetic process [TAS]
- innate immune response [TAS]
- intracellular transport of virus [TAS]
- ion transmembrane transport [TAS]
- mRNA metabolic process [TAS]
- membrane organization [TAS]
- mitotic cell cycle [TAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- negative regulation of type I interferon production [TAS]
- negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [TAS]
- positive regulation of NF-kappaB transcription factor activity [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of transcription from RNA polymerase II promoter [TAS]
- positive regulation of type I interferon production [TAS]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- protein polyubiquitination [TAS]
- regulation of apoptotic process [TAS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- regulation of type I interferon production [TAS]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- small molecule metabolic process [TAS]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- transmembrane transport [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral protein processing [TAS]
- virion assembly [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Site-specific Interaction Mapping of Phosphorylated Ubiquitin to Uncover Parkin Activation.
Damaged mitochondria are eliminated through autophagy machinery. A cytosolic E3 ubiquitin ligase Parkin, a gene product mutated in familial Parkinsonism, is essential for this pathway. Recent progress has revealed that phosphorylation of both Parkin and ubiquitin at Ser(65) by PINK1 are crucial for activation and recruitment of Parkin to the damaged mitochondria. However, the mechanism by which phosphorylated ubiquitin associates ... [more]
J. Biol. Chem. Oct. 16, 2015; 290(42);25199-211 [Pubmed: 26260794]
Throughput
- Low Throughput
Additional Notes
- #LPPI
- Chemically cross-linked with BPA
- Likely protein-protein interaction
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBC PARK2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 3401884 | |
| PARK2 UBC | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1510636 |
Curated By
- BioGRID