EGLN1
Gene Ontology Biological Process
- cellular response to hypoxia [TAS]
- negative regulation of cAMP catabolic process [ISS]
- negative regulation of cyclic-nucleotide phosphodiesterase activity [ISS]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- oxygen homeostasis [IDA]
- peptidyl-proline hydroxylation to 4-hydroxy-L-proline [IDA]
- regulation of angiogenesis [ISS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- response to hypoxia [IDA]
- response to nitric oxide [IDA]
Gene Ontology Molecular Function- enzyme binding [ISS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]
- peptidyl-proline 4-dioxygenase activity [IDA]
- peptidyl-proline dioxygenase activity [TAS]
- protein binding [IPI]
- enzyme binding [ISS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]
- peptidyl-proline 4-dioxygenase activity [IDA]
- peptidyl-proline dioxygenase activity [TAS]
- protein binding [IPI]
GPLD1
Gene Ontology Biological Process
- GPI anchor release [IDA, IMP]
- cell migration involved in sprouting angiogenesis [IMP]
- cellular response to calcium ion [IDA]
- cellular response to cholesterol [IMP]
- cellular response to drug [IDA]
- cellular response to insulin stimulus [IDA]
- cellular response to pH [IDA]
- cellular response to triglyceride [IMP]
- chondrocyte differentiation [ISS]
- complement receptor mediated signaling pathway [IDA]
- hematopoietic stem cell migration [TAS]
- hematopoietic stem cell migration to bone marrow [TAS]
- insulin receptor signaling pathway [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of triglyceride catabolic process [ISS]
- ossification [ISS]
- phosphatidylcholine metabolic process [ISS, TAS]
- positive regulation of alkaline phosphatase activity [ISS]
- positive regulation of apoptotic process [IDA]
- positive regulation of cytolysis [IDA]
- positive regulation of endothelial cell migration [IMP]
- positive regulation of glucose metabolic process [ISS]
- positive regulation of high-density lipoprotein particle clearance [ISS]
- positive regulation of insulin secretion involved in cellular response to glucose stimulus [ISS]
- positive regulation of membrane protein ectodomain proteolysis [IDA]
- positive regulation of secretion [ISS]
- positive regulation of triglyceride biosynthetic process [ISS]
- regulation of cellular response to insulin stimulus [IDA]
- response to glucose [IDA]
- transepithelial transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The pleckstrin homology domain of phospholipase D1 accelerates EGFR endocytosis by increasing the expression of the Rab5 effector, rabaptin-5.
Endocytosis is differentially regulated by hypoxia-inducible factor-1α (HIF-1α) and phospholipase D (PLD). However, the relationship between HIF-1α and PLD in endocytosis is unknown. HIF-1α is degraded through the prolyl hydroxylase (PHD)/von Hippel-Lindau (VHL) ubiquitination pathway in an oxygen-dependent manner. Here, we show that PLD1 recovers the decrease in epidermal growth factor receptor (EGFR) endocytosis induced by HIF-1α independent of lipase ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 3
Curated By
- BioGRID