BAIT

NPHS2

AI790225, PDCN, SRN1

nephrosis 2, podocin

GO Process (1)

GO Function (1)

GO Component (7)

Gene Ontology Biological Process

actin cytoskeleton reorganization [ISO]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

cell-cell junction [ISO]

extracellular vesicular exosome [ISO]

intrinsic component of the cytoplasmic side of the plasma membrane [IDA, ISO]

membrane raft [ISO]

plasma membrane [IDA]

protein complex [ISO]

slit diaphragm [IDA, ISO]

CRISPR Database MGI Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

PREY

TFRC

2610028K12Rik, AI195355, AI426448, AU015758, CD71, E430033M20Rik, Mtvr-1, Mtvr1, TFR, TFR1, TR, Trfr, p90

transferrin receptor

GO Process (7)

GO Function (8)

GO Component (17)

Gene Ontology Biological Process

cellular iron ion homeostasis [IMP]

cellular response to drug [ISO]

iron ion import [ISO]

iron ion transmembrane transport [TAS]

osteoclast differentiation [IMP]

positive regulation of bone resorption [IDA]

receptor-mediated endocytosis [ISO]

Gene Ontology Molecular Function

Hsp70 protein binding [ISO]
chaperone binding [ISO]
double-stranded RNA binding [ISO]
identical protein binding [ISO]
iron ion transmembrane transporter activity [TAS]
poly(A) RNA binding [ISO]
protein binding [IPI]
transferrin receptor activity [ISO, TAS]

Gene Ontology Cellular Component

blood microparticle [ISO]

cell surface [IDA, ISO]

coated pit [IDA, ISO]

cytoplasmic membrane-bounded vesicle [ISO]

endosome [IDA, ISO]

external side of plasma membrane [IDA]

extracellular region [ISO]

extracellular space [ISO]

extracellular vesicular exosome [IDA, ISO]

intracellular membrane-bounded organelle [ISO]

mitochondrion [ISO]

perinuclear region of cytoplasm [IDA]

plasma membrane [IDA, ISO]

recycling endosome [IDA, ISO]

recycling endosome membrane [ISO]

CRISPR Database VEGA MGI Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes.

Rinschen MM, Bharill P, Wu X, Kohli P, Reinert MJ, Kretz O, Saez I, Schermer B, Hoehne M, Bartram MP, Aravamudhan S, Brooks BR, Vilchez D, Huber TB, Mueller RU, Krueger M, Benzing T

The PHB-domain protein podocin maintains the renal filtration barrier and its mutation is an important cause of hereditary nephrotic syndrome. Podocin and its Caenorhabditis elegans orthologue MEC-2 have emerged as key components of mechanosensitive membrane protein signalling complexes. Whereas podocin resides at a specialized cell junction at the podocyte slit diaphragm, MEC-2 is found in neurons required for touch sensitivity. ... [more]

Hum. Mol. Genet. Apr. 01, 2016; 25(7);1328-44 [Pubmed: 26792178]

Throughput

High Throughput

Additional Notes

High confidence interaction
Interactor of FLAG-tagged podocin expressed in podocytes

Curated By

BioGRID