BAIT
NPHS2
AI790225, PDCN, SRN1
nephrosis 2, podocin
GO Process (1)
GO Function (1)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ATP2A2
9530097L16Rik, D5Wsu150e, SERCA2, SERCA2B, mKIAA4195
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
GO Process (22)
GO Function (9)
GO Component (13)
Gene Ontology Biological Process
- ATP catabolic process [IDA, ISO]
- ER-nucleus signaling pathway [IMP]
- T-tubule organization [IMP]
- calcium ion import into sarcoplasmic reticulum [ISO]
- calcium ion transmembrane transport [IDA, ISO]
- calcium ion transport [ISO]
- calcium ion transport from cytosol to endoplasmic reticulum [ISO]
- cellular calcium ion homeostasis [IDA, IGI, IMP, ISO]
- endoplasmic reticulum calcium ion homeostasis [ISO]
- negative regulation of heart contraction [IGI]
- organelle organization [IMP]
- positive regulation of endoplasmic reticulum calcium ion concentration [ISO]
- regulation of calcium ion-dependent exocytosis of neurotransmitter [IMP]
- regulation of cardiac muscle cell action potential involved in regulation of contraction [ISO]
- regulation of cardiac muscle cell membrane potential [ISO]
- regulation of cardiac muscle contraction by calcium ion signaling [ISO]
- regulation of muscle contraction [TAS]
- regulation of the force of heart contraction [IGI]
- relaxation of cardiac muscle [ISO]
- response to peptide hormone [ISO]
- sarcoplasmic reticulum calcium ion transport [IMP, ISO]
- transition between fast and slow fiber [IDA]
Gene Ontology Molecular Function- ATP binding [ISO]
- S100 protein binding [ISO]
- calcium ion binding [ISO]
- calcium-transporting ATPase activity [IDA, ISO]
- calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential [ISO]
- enzyme binding [IPI, ISO]
- lutropin-choriogonadotropic hormone receptor binding [ISO]
- protein C-terminus binding [IPI, ISO]
- protein binding [IPI]
- ATP binding [ISO]
- S100 protein binding [ISO]
- calcium ion binding [ISO]
- calcium-transporting ATPase activity [IDA, ISO]
- calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential [ISO]
- enzyme binding [IPI, ISO]
- lutropin-choriogonadotropic hormone receptor binding [ISO]
- protein C-terminus binding [IPI, ISO]
- protein binding [IPI]
Gene Ontology Cellular Component
- calcium ion-transporting ATPase complex [ISO]
- cell [IGI]
- endoplasmic reticulum [IDA, ISO]
- endoplasmic reticulum membrane [ISO]
- extrinsic component of cytoplasmic side of plasma membrane [ISO]
- intercalated disc [ISO]
- longitudinal sarcoplasmic reticulum [ISO]
- membrane [IDA, ISO]
- perinuclear region of cytoplasm [ISO]
- protein complex [ISO]
- ribbon synapse [IDA]
- sarcoplasmic reticulum [IDA, ISO, TAS]
- vesicle membrane [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes.
The PHB-domain protein podocin maintains the renal filtration barrier and its mutation is an important cause of hereditary nephrotic syndrome. Podocin and its Caenorhabditis elegans orthologue MEC-2 have emerged as key components of mechanosensitive membrane protein signalling complexes. Whereas podocin resides at a specialized cell junction at the podocyte slit diaphragm, MEC-2 is found in neurons required for touch sensitivity. ... [more]
Hum. Mol. Genet. Apr. 01, 2016; 25(7);1328-44 [Pubmed: 26792178]
Throughput
- High Throughput
Additional Notes
- High confidence interaction
- Interactor of FLAG-tagged podocin expressed in podocytes
Curated By
- BioGRID