BAIT

NPHS2

AI790225, PDCN, SRN1
nephrosis 2, podocin
GO Process (1)
GO Function (1)
GO Component (7)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

TUBB2B

2410129E14Rik, brdp
tubulin, beta 2B class IIB
GO Process (1)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes.

Rinschen MM, Bharill P, Wu X, Kohli P, Reinert MJ, Kretz O, Saez I, Schermer B, Hoehne M, Bartram MP, Aravamudhan S, Brooks BR, Vilchez D, Huber TB, Mueller RU, Krueger M, Benzing T

The PHB-domain protein podocin maintains the renal filtration barrier and its mutation is an important cause of hereditary nephrotic syndrome. Podocin and its Caenorhabditis elegans orthologue MEC-2 have emerged as key components of mechanosensitive membrane protein signalling complexes. Whereas podocin resides at a specialized cell junction at the podocyte slit diaphragm, MEC-2 is found in neurons required for touch sensitivity. ... [more]

Hum. Mol. Genet. Apr. 01, 2016; 25(7);1328-44 [Pubmed: 26792178]

Throughput

  • High Throughput

Additional Notes

  • High confidence interaction
  • Interactor of podocin expressed in native glomeruli

Curated By

  • BioGRID