Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes.

Rinschen MM, Bharill P, Wu X, Kohli P, Reinert MJ, Kretz O, Saez I, Schermer B, Hoehne M, Bartram MP, Aravamudhan S, Brooks BR, Vilchez D, Huber TB, Mueller RU, Krueger M, Benzing T

The PHB-domain protein podocin maintains the renal filtration barrier and its mutation is an important cause of hereditary nephrotic syndrome. Podocin and its Caenorhabditis elegans orthologue MEC-2 have emerged as key components of mechanosensitive membrane protein signalling complexes. Whereas podocin resides at a specialized cell junction at the podocyte slit diaphragm, MEC-2 is found in neurons required for touch sensitivity. ... [more]

Hum. Mol. Genet. Apr. 01, 2016; 25(7);1328-44 [Pubmed: 26792178]

Throughput

  • High Throughput

Additional Notes

  • High confidence interaction
  • Interactor of podocin expressed in native glomeruli

Curated By

  • BioGRID