A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

gp78 functions downstream of Hrd1 to promote degradation of misfolded proteins of the endoplasmic reticulum.

Zhang T, Xu Y, Liu Y, Ye Y

Eukaryotic cells eliminate misfolded proteins from the endoplasmic reticulum (ER) via a conserved process termed ER-associated degradation (ERAD). Central regulators of the ERAD system are membrane-bound ubiquitin ligases, which are thought to channel misfolded proteins through the ER membrane during retrotranslocation. Hrd1 and gp78 are mammalian ubiquitin ligases homologous to Hrd1p, an ubiquitin ligase essential for ERAD in Saccharomyces cerevisiae. ... [more]

Mol. Biol. Cell Dec. 01, 2015; 26(24);4438-50 [Pubmed: 26424800]

Throughput

Low Throughput

Ontology Terms

phenotype: abnormal protein level (MP:0008469)

Additional Notes

CRISPR/Cas9
the accumulation of ubiquitinated MHC 1-147 and retrotranslocated deglycosylated heavy chain molecules seen in a Bag6 or gp78 knockdown condition was reversed upon depletion of Hrd1 as measured by the split-GFP assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AMFR SYVN1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Ballar P (2010)	Low	-	BioGRID	-
AMFR SYVN1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Wang L (2022)	Low	-	BioGRID	-
SYVN1 AMFR	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zhang T (2015)	Low	-	BioGRID	-
AMFR SYVN1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zhang T (2015)	Low	-	BioGRID	-
AMFR SYVN1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zhong Y (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AMFR

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]receptor activity [IPI]ubiquitin protein ligase activity [IDA]ubiquitin-protein transferase activity [IDA, IMP]

Gene Ontology Cellular Component

SYVN1