BAIT

MCM5

CDC46, P1-CDC46, RP5-824I19__A.1
minichromosome maintenance complex component 5
GO Process (4)
GO Function (1)
GO Component (4)
Homo sapiens
PREY

TUBB3

CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4
tubulin, beta 3 class III
GO Process (4)
GO Function (1)
GO Component (4)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative Proteomics Reveals Dynamic Interactions of the Minichromosome Maintenance Complex (MCM) in the Cellular Response to Etoposide Induced DNA Damage.

Drissi R, Dubois ML, Douziech M, Boisvert FM

The minichromosome maintenance complex (MCM) proteins are required for processive DNA replication and are a target of S-phase checkpoints. The eukaryotic MCM complex consists of six proteins (MCM2-7) that form a heterohexameric ring with DNA helicase activity, which is loaded on chromatin to form the pre-replication complex. Upon entry in S phase, the helicase is activated and opens the DNA ... [more]

Mol. Cell Proteomics Jul. 01, 2015; 14(7);2002-13 [Pubmed: 25963833]

Throughput

  • High Throughput

Curated By

  • BioGRID