An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative Proteomics Reveals Dynamic Interactions of the Minichromosome Maintenance Complex (MCM) in the Cellular Response to Etoposide Induced DNA Damage.

Drissi R, Dubois ML, Douziech M, Boisvert FM

The minichromosome maintenance complex (MCM) proteins are required for processive DNA replication and are a target of S-phase checkpoints. The eukaryotic MCM complex consists of six proteins (MCM2-7) that form a heterohexameric ring with DNA helicase activity, which is loaded on chromatin to form the pre-replication complex. Upon entry in S phase, the helicase is activated and opens the DNA ... [more]

Mol. Cell Proteomics Jul. 01, 2015; 14(7);2002-13 [Pubmed: 25963833]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MCM2 MCM2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Kneissl M (2003)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MCM2

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]protein binding [IPI]

Gene Ontology Cellular Component