BAIT
RNF165
ARKL2, RNF111L2
ring finger protein 165
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
UBE2N
HEL-S-71, UBC13, UBCHBEN; UBC13, UbcH-ben, UbcH13
ubiquitin-conjugating enzyme E2N
GO Process (30)
GO Function (7)
GO Component (8)
Gene Ontology Biological Process
- DNA double-strand break processing [IMP]
- Fc-epsilon receptor signaling pathway [TAS]
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- T cell receptor signaling pathway [IMP, TAS]
- cellular protein modification process [TAS]
- cytokine-mediated signaling pathway [TAS]
- double-strand break repair via homologous recombination [IMP]
- histone ubiquitination [IMP]
- innate immune response [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- positive regulation of DNA repair [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP, TAS]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of histone modification [IMP]
- positive regulation of ubiquitin-protein transferase activity [IMP]
- postreplication repair [IMP]
- protein K63-linked ubiquitination [IDA]
- protein ubiquitination [IMP, TAS]
- proteolysis [TAS]
- regulation of DNA repair [TAS]
- regulation of histone ubiquitination [IMP]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Secondary ubiquitin-RING docking enhances Arkadia and Ark2C E3 ligase activity.
RING-domain E3 ligases enhance transfer of ubiquitin to substrate proteins by stabilizing the RING-bound thioester-linked E2∼ubiquitin conjugate in a defined conformation that primes the active site for nucleophilic attack. Here we report that the monomeric RING domains from the human E3 ligases Arkadia and Ark2C bind directly to free ubiquitin with an affinity comparable to that of other dedicated ubiquitin-binding ... [more]
Nat. Struct. Mol. Biol. Jan. 01, 2016; 23(1);45-52 [Pubmed: 26656854]
Throughput
- Low Throughput
Additional Notes
- E2:MMS2-UBC13
- forms poly-ubiquitin in vitro in the presence of Ubc13-MMS2
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBE2N RNF165 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID