Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

The proximity ligation assay reveals that at DNA double-strand breaks WRAP53Î² associates with Î³H2AX and controls interactions between RNF8 and MDC1.

Rassoolzadeh H, Coucoravas C, Farnebo M

We recently demonstrated that WRAP53Î² acts as a key regulator of ubiquitin-dependent repair of DNA double-strand breaks. Here, we applied the proximity ligation assay (PLA) to show that at such breaks WRAP53Î² accumulates in close proximity to Î³H2AX and, furthermore as demonstrated by their co-immunoprecipitation (IP) binds to Î³H2AX, in a manner dependent on the ATM and ATR kinases. Moreover, ... [more]

Nucleus Sep. 03, 2015; 6(5);417-24 [Pubmed: 26734725]

Throughput

Low Throughput

Additional Notes

in situ PLA

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RNF8 MDC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Henriksson S (2014)	Low	-	BioGRID	-
MDC1 RNF8	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Henriksson S (2014)	Low	-	BioGRID	-
MDC1 RNF8	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Kolas NK (2007)	Low	-	BioGRID	-
RNF8 MDC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Lee KY (2017)	Low	-	BioGRID	2469463
RNF8 MDC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Mailand N (2007)	Low	-	BioGRID	-
MDC1 RNF8	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Mailand N (2007)	Low	-	BioGRID	-
RNF8 MDC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Watanabe S (2013)	Low	-	BioGRID	-
RNF8 MDC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Huen MS (2007)	Low	-	BioGRID	-
RNF8 MDC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Kolas NK (2007)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MDC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

FHA domain binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

RNF8

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]histone binding [IDA]protein binding [IPI]protein homodimerization activity [IDA]ubiquitin protein ligase binding [IPI]ubiquitin-protein transferase activity [IDA]zinc ion binding [IDA]