NOX4
Gene Ontology Biological Process
- aging [IEP]
- bone resorption [ISO]
- cardiac muscle cell differentiation [ISO]
- cell aging [ISO, ISS]
- cell morphogenesis [ISO, ISS]
- cellular response to cAMP [IEP]
- cellular response to gamma radiation [IEP]
- cellular response to transforming growth factor beta stimulus [IEP]
- homocysteine metabolic process [ISO]
- negative regulation of cell proliferation [ISO, ISS]
- oxidation-reduction process [ISO, ISS]
- positive regulation of DNA biosynthetic process [ISO]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of MAP kinase activity [ISO]
- positive regulation of apoptotic process [IMP]
- positive regulation of protein kinase B signaling [ISO]
- positive regulation of reactive oxygen species metabolic process [IMP]
- positive regulation of smooth muscle cell migration [IMP]
- positive regulation of stress fiber assembly [IMP]
- reactive oxygen species metabolic process [ISO, ISS]
- response to hypoxia [IEP]
- superoxide anion generation [ISO, ISS]
- superoxide metabolic process [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HSPA1A
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- DNA repair [ISO]
- binding of sperm to zona pellucida [ISO]
- cellular heat acclimation [ISO]
- cellular response to heat [ISO]
- defense response [IDA]
- mRNA catabolic process [ISO, ISS]
- negative regulation of apoptotic process [IDA, ISO]
- negative regulation of cell death [ISO]
- negative regulation of cell growth [ISO, ISS]
- negative regulation of cell proliferation [ISO, ISS]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA, ISO]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [ISO]
- negative regulation of inclusion body assembly [ISO]
- negative regulation of myeloid cell apoptotic process [IDA]
- negative regulation of protein ubiquitination [ISO]
- negative regulation of vasoconstriction [IMP]
- positive regulation of T cell mediated cytotoxicity [IDA]
- positive regulation of erythrocyte differentiation [ISO]
- protein refolding [ISO]
- regulation of cell death [ISO]
- response to heat [ISO]
- response to unfolded protein [IMP, ISO, ISS]
- telomere maintenance [ISO]
Gene Ontology Molecular Function- ATP binding [ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [ISO]
- G-protein coupled receptor binding [ISO]
- NF-kappaB binding [IPI]
- double-stranded RNA binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protease binding [IPI]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein binding involved in protein folding [ISO]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [ISO]
- G-protein coupled receptor binding [ISO]
- NF-kappaB binding [IPI]
- double-stranded RNA binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protease binding [IPI]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein binding involved in protein folding [ISO]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [ISO]
Gene Ontology Cellular Component
- COP9 signalosome [ISO]
- aggresome [ISO]
- apical plasma membrane [IDA]
- basolateral plasma membrane [IDA]
- blood microparticle [ISO]
- cell body [ISO]
- centriole [ISO]
- cytoplasm [ISO, ISS]
- cytosol [IDA, ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- inclusion body [ISO]
- intracellular [ISO]
- mitochondrion [ISO]
- nuclear speck [ISO, ISS]
- nucleus [IDA, ISO, ISS]
- perinuclear region of cytoplasm [ISO, ISS]
- protein complex [IDA]
- ribonucleoprotein complex [ISO]
- ubiquitin ligase complex [ISO]
- vesicle [ISO]
- zona pellucida receptor complex [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Heat Shock Protein 70 and CHIP Promote Nox4 Ubiquitination and Degradation within the Losartan Antioxidative Effect in Proximal Tubule Cells.
Angiotensin II/Angiotensin II type 1 receptor (AT1R) effects are dependent on ROS production stimulated by NADPH oxidase activation. Hsp70 regulates a diverse set of signaling pathways through their interactions with proteins. CHIP is a E3 ubiquitin ligase that targets proteins for polyubiquitination and degradation.We study whether Hsp70/CHIP contribute to the negative regulation of Nox4 after AT1R blockage.Primary culture of proximal ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 2
Curated By
- BioGRID