BAIT

USP12

UBH1, USP12L1
ubiquitin specific peptidase 12
UPS Project
GO Process (1)
GO Function (4)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

CBLB

Cbl-b, RNF56, Nbla00127
Cbl proto-oncogene B, E3 ubiquitin protein ligase
UPS Project
GO Process (2)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.

Jahan AS, Lestra M, Swee LK, Fan Y, Lamers MM, Tafesse FG, Theile CS, Spooner E, Bruzzone R, Ploegh HL, Sanyal S

Posttranslational modifications are central to the spatial and temporal regulation of protein function. Among others, phosphorylation and ubiquitylation are known to regulate proximal T-cell receptor (TCR) signaling. Here we used a systematic and unbiased approach to uncover deubiquitylating enzymes (DUBs) that participate during TCR signaling in primary mouse T lymphocytes. Using a C-terminally modified vinyl methyl ester variant of ubiquitin ... [more]

Proc. Natl. Acad. Sci. U.S.A. Feb. 09, 2016; 113(6);E705-14 [Pubmed: 26811477]

Throughput

  • High Throughput

Additional Notes

  • BioID

Curated By

  • BioGRID