An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.

Jahan AS, Lestra M, Swee LK, Fan Y, Lamers MM, Tafesse FG, Theile CS, Spooner E, Bruzzone R, Ploegh HL, Sanyal S

Posttranslational modifications are central to the spatial and temporal regulation of protein function. Among others, phosphorylation and ubiquitylation are known to regulate proximal T-cell receptor (TCR) signaling. Here we used a systematic and unbiased approach to uncover deubiquitylating enzymes (DUBs) that participate during TCR signaling in primary mouse T lymphocytes. Using a C-terminally modified vinyl methyl ester variant of ubiquitin ... [more]

Proc. Natl. Acad. Sci. U.S.A. Feb. 09, 2016; 113(6);E705-14 [Pubmed: 26811477]

Throughput

High Throughput

Additional Notes

Deubiquitylating enzymes retrieved by HA-Ub-VME

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UBC USP4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zhang X (2017)	High	-	BioGRID	2297339

Curated By

BioGRID

Interaction Summary

UBC

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [ISO]protease binding [ISO]protein binding [IPI]

Gene Ontology Cellular Component

USP4

Gene Ontology Biological Process

Gene Ontology Molecular Function

adenosine receptor binding [ISO]cysteine-type endopeptidase activity [IBA]identical protein binding [ISO]protein binding [IPI]ubiquitin thiolesterase activity [IBA, ISO]ubiquitin-specific protease activity [IBA, ISO]