BAIT

UBC

2700054O04Rik, AI194771, Rps27a, TI-225, Uba52, Ubb
ubiquitin C
GO Process (1)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

OTUB1

AI850305
OTU domain, ubiquitin aldehyde binding 1
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.

Jahan AS, Lestra M, Swee LK, Fan Y, Lamers MM, Tafesse FG, Theile CS, Spooner E, Bruzzone R, Ploegh HL, Sanyal S

Posttranslational modifications are central to the spatial and temporal regulation of protein function. Among others, phosphorylation and ubiquitylation are known to regulate proximal T-cell receptor (TCR) signaling. Here we used a systematic and unbiased approach to uncover deubiquitylating enzymes (DUBs) that participate during TCR signaling in primary mouse T lymphocytes. Using a C-terminally modified vinyl methyl ester variant of ubiquitin ... [more]

Proc. Natl. Acad. Sci. U.S.A. Feb. 09, 2016; 113(6);E705-14 [Pubmed: 26811477]

Throughput

  • High Throughput

Additional Notes

  • Deubiquitylating enzymes retrieved by HA-Ub-VME

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBC OTUB1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

High-BioGRID
2297377

Curated By

  • BioGRID