UBC
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CYLD
Gene Ontology Biological Process
- necroptotic process [IGI]
- negative regulation of NF-kappaB import into nucleus [ISO]
- negative regulation of NF-kappaB transcription factor activity [IBA, ISO]
- negative regulation of T cell differentiation [IDA]
- negative regulation of canonical Wnt signaling pathway [ISO]
- positive regulation of extrinsic apoptotic signaling pathway [IBA, ISO]
- protein K63-linked deubiquitination [IBA, ISO]
- protein deubiquitination [IMP]
- proteolysis [IBA]
- regulation of intrinsic apoptotic signaling pathway [IBA, ISO]
- regulation of microtubule cytoskeleton organization [ISO]
- regulation of mitotic cell cycle [IBA, ISO]
- ripoptosome assembly involved in necroptotic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.
Posttranslational modifications are central to the spatial and temporal regulation of protein function. Among others, phosphorylation and ubiquitylation are known to regulate proximal T-cell receptor (TCR) signaling. Here we used a systematic and unbiased approach to uncover deubiquitylating enzymes (DUBs) that participate during TCR signaling in primary mouse T lymphocytes. Using a C-terminally modified vinyl methyl ester variant of ubiquitin ... [more]
Throughput
- High Throughput
Additional Notes
- Deubiquitylating enzymes retrieved by HA-Ub-VME
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBC CYLD | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | High | - | BioGRID | 2297332 |
Curated By
- BioGRID