RHOU
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PLEK
Gene Ontology Biological Process
- actin cytoskeleton reorganization [IDA]
- blood coagulation [TAS]
- cell projection organization [IDA]
- cortical actin cytoskeleton organization [IDA]
- hematopoietic progenitor cell differentiation [IEP]
- integrin-mediated signaling pathway [IDA]
- negative regulation of G-protein coupled receptor protein signaling pathway [IDA]
- negative regulation of calcium-mediated signaling [NAS]
- negative regulation of inositol phosphate biosynthetic process [IDA]
- phosphatidylinositol metabolic process [IDA]
- phospholipase C-inhibiting G-protein coupled receptor signaling pathway [IDA]
- platelet activation [TAS]
- platelet aggregation [IMP, ISS]
- platelet degranulation [IEP, TAS]
- positive regulation of actin filament bundle assembly [IDA]
- positive regulation of actin filament depolymerization [IDA]
- positive regulation of inositol-polyphosphate 5-phosphatase activity [IDA]
- positive regulation of integrin activation [ISS]
- positive regulation of platelet activation [ISS]
- protein kinase C signaling [ISS]
- protein secretion by platelet [ISS]
- regulation of cell diameter [IDA]
- ruffle organization [IDA]
- thrombin receptor signaling pathway [IDA]
- vesicle docking involved in exocytosis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
PAK4 promotes kinase-independent stabilization of RhoU to modulate cell adhesion.
P21-activated kinase 4 (PAK4) is a Cdc42 effector protein thought to regulate cell adhesion disassembly in a kinase-dependent manner. We found that PAK4 expression is significantly higher in high-grade human breast cancer patient samples, whereas depletion of PAK4 modifies cell adhesion dynamics of breast cancer cells. Surprisingly, systematic analysis of PAK4 functionality revealed that PAK4-driven adhesion turnover is neither dependent ... [more]
Throughput
- High Throughput
Additional Notes
- Table S1
- identified via protein microarray
Curated By
- BioGRID