BAIT

RHOU

ARHU, CDC42L1, G28K, WRCH1, hG28K, SB128

ras homolog family member U

GO Process (4)

GO Function (1)

GO Component (2)

Gene Ontology Biological Process

cytoskeleton organization [IMP]

regulation of cell shape [IMP]

regulation of small GTPase mediated signal transduction [TAS]

small GTPase mediated signal transduction [TAS]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

plasma membrane [TAS]

CRISPR Database OMIM HGNC Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

PSMA4

HC9, HsT17706, PSC9

proteasome (prosome, macropain) subunit, alpha type, 4

GO Process (21)

GO Function (1)

GO Component (10)

Gene Ontology Biological Process

DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [TAS]

G1/S transition of mitotic cell cycle [TAS]

RNA metabolic process [TAS]

anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]

antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]

antigen processing and presentation of peptide antigen via MHC class I [TAS]

apoptotic process [TAS]

cellular nitrogen compound metabolic process [TAS]

gene expression [TAS]

mRNA metabolic process [TAS]

mitotic cell cycle [TAS]

negative regulation of apoptotic process [TAS]

negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

protein polyubiquitination [TAS]

regulation of apoptotic process [TAS]

regulation of cellular amino acid metabolic process [TAS]

regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

small molecule metabolic process [TAS]

viral process [TAS]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

cytoplasm [IDA]

cytoplasmic mRNA processing body [ISS]

extracellular vesicular exosome [IDA]

intracellular membrane-bounded organelle [IDA]

nucleoplasm [TAS]

plasma membrane [IDA]

proteasome complex [IDA]

proteasome core complex [ISS]

CRISPR Database OMIM HGNC Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

PAK4 promotes kinase-independent stabilization of RhoU to modulate cell adhesion.

Dart AE, Box GM, Court W, Gale ME, Brown JP, Pinder SE, Eccles SA, Wells CM

P21-activated kinase 4 (PAK4) is a Cdc42 effector protein thought to regulate cell adhesion disassembly in a kinase-dependent manner. We found that PAK4 expression is significantly higher in high-grade human breast cancer patient samples, whereas depletion of PAK4 modifies cell adhesion dynamics of breast cancer cells. Surprisingly, systematic analysis of PAK4 functionality revealed that PAK4-driven adhesion turnover is neither dependent ... [more]

J. Cell Biol. Nov. 23, 2015; 211(4);863-79 [Pubmed: 26598620]

Throughput

High Throughput

Additional Notes

Table S1
identified via protein microarray

Curated By

BioGRID