An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Signal Transduction and Intracellular Trafficking by the Interleukin 36 Receptor.

Saha SS, Singh D, Raymond EL, Ganesan R, Caviness G, Grimaldi C, Woska JR, Mennerich D, Brown SE, Mbow ML, Kao CC

Improper signaling of the IL-36 receptor (IL-36R), a member of the IL-1 receptor family, has been associated with various inflammation-associated diseases. However, the requirements for IL-36R signal transduction remain poorly characterized. This work seeks to define the requirements for IL-36R signaling and intracellular trafficking. In the absence of cognate agonists, IL-36R was endocytosed and recycled to the plasma membrane. In ... [more]

J. Biol. Chem. Sep. 25, 2015; 290(39);23997-4006 [Pubmed: 26269592]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TOLLIP

Gene Ontology Biological Process

Gene Ontology Molecular Function

Toll-like receptor binding [IPI]kinase binding [IPI]protein binding [IPI]signal transducer activity [NAS]

Gene Ontology Cellular Component

IL1RL2