BAIT

ITCH

ADMFD, AIF4, AIP4, NAPP1, dJ468O1.1
itchy E3 ubiquitin protein ligase
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Multiple Src Homology 3 Binding to the Ubiquitin Ligase Itch Conserved Proline-Rich Region.

Desrochers G, Lussier-Price M, Omichinski JG, Angers A

Itch is a member of the C2-WW-HECT (CWH) family of ubiquitin ligases involved in the control of inflammatory signaling pathways, several transcription factors, and sorting of surface receptors to the degradative pathway. In addition to these common domains, Itch also contains a conserved proline-rich region (PRR) allowing its interaction with Src homology 3 (SH3) domain-containing proteins. This region is composed ... [more]

Biochemistry Dec. 22, 2015; 54(50);7345-54 [Pubmed: 26613292]

Throughput

  • Low Throughput

Additional Notes

  • Figure 4
  • ITC

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ITCH PACSIN1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
1513638
ITCH PACSIN1
Far Western
Far Western

An interaction is detected between a protein immobilized on a membrane and a purified protein probe.

Low-BioGRID
1513642
ITCH PACSIN1
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
1513633
ITCH PACSIN1
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Low-BioGRID
2449211

Curated By

  • BioGRID