KCNA5
Gene Ontology Biological Process
- atrial cardiac muscle cell action potential [ISO]
- membrane hyperpolarization [ISO]
- membrane repolarization during cardiac muscle cell action potential [ISO]
- negative regulation of cytosolic calcium ion concentration [ISO]
- negative regulation of potassium ion transport [IDA]
- positive regulation of G1/S transition of mitotic cell cycle [ISO]
- positive regulation of myoblast proliferation [ISO]
- potassium ion export [ISO]
- potassium ion homeostasis [ISO]
- potassium ion transmembrane transport [IBA, IDA]
- potassium ion transport [IDA, ISO]
- protein oligomerization [ISO]
- regulation of atrial cardiac muscle cell membrane repolarization [ISO]
- regulation of heart rate by cardiac conduction [ISO]
- regulation of ion transmembrane transport [IBA]
- regulation of membrane potential [IGI, ISO]
- regulation of potassium ion transport [ISO]
- regulation of vasoconstriction [IMP]
Gene Ontology Molecular Function- alpha-actinin binding [ISO]
- delayed rectifier potassium channel activity [IBA, ISO]
- outward rectifier potassium channel activity [ISO]
- potassium channel inhibitor activity [IDA]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [ISO]
- voltage-gated potassium channel activity [IDA]
- voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization [ISO]
- alpha-actinin binding [ISO]
- delayed rectifier potassium channel activity [IBA, ISO]
- outward rectifier potassium channel activity [ISO]
- potassium channel inhibitor activity [IDA]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [ISO]
- voltage-gated potassium channel activity [IDA]
- voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- Z disc [ISO]
- cytosol [ISO]
- endoplasmic reticulum [ISO]
- integral component of membrane [IBA]
- integral component of plasma membrane [IC]
- intercalated disc [IDA, ISO]
- intracellular canaliculus [ISO]
- membrane [IDA]
- membrane raft [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [IDA, ISO]
- voltage-gated potassium channel complex [ISO]
HSPA8
Gene Ontology Biological Process
- ATP catabolic process [IDA, ISO]
- chaperone mediated protein folding requiring cofactor [IGI, ISO]
- chaperone-mediated protein folding [ISO]
- clathrin coat disassembly [IGI]
- negative regulation of fibril organization [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of mRNA splicing, via spliceosome [IMP]
- protein folding [IDA]
- protein refolding [ISO]
- regulation of cell cycle [IDA]
Gene Ontology Molecular Function- ADP binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [IDA, ISO]
- G-protein coupled receptor binding [ISO]
- MHC class II protein complex binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- receptor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [IPI, ISO]
- ADP binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [IDA, ISO]
- G-protein coupled receptor binding [ISO]
- MHC class II protein complex binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- receptor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [IPI, ISO]
Gene Ontology Cellular Component
- Prp19 complex [ISO]
- blood microparticle [ISO]
- cytoplasm [ISO]
- cytosol [IDA, ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [IDA, ISO]
- focal adhesion [ISO]
- intracellular [IDA]
- membrane [ISO]
- myelin sheath [IDA]
- neuron projection [ISO]
- neuronal cell body [ISO]
- nucleus [ISO]
- protein complex [ISO]
- ribonucleoprotein complex [ISO]
- synaptic vesicle [ISO]
- terminal bouton [ISO]
- ubiquitin ligase complex [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
E3 ligase CHIP and Hsc70 regulate Kv1.5 protein expression and function in mammalian cells.
Kv1.5 confers ultra-rapid delayed-rectifier potassium channel current (IKur) which contributes to repolarization of the atrial action potential. Kv1.5 proteins, degraded via the ubiquitin-proteasome pathway, decreased in some atrial fibrillation patients. Carboxyl-terminus heat shock cognate 70-interacting protein (CHIP), an E3 ubiquitin ligase, is known to ubiquitinate short-lived proteins. Here, we investigated the roles of CHIP in Kv1.5 degradation to provide insights ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure S6
Curated By
- BioGRID