PREY

PSMC1

AI325227, P26s4, Rpt2/S4, S4, rpt2
protease (prosome, macropain) 26S subunit, ATPase 1
GO Process (0)
GO Function (2)
GO Component (8)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

TOPORS, a Dual E3 Ubiquitin and Sumo1 Ligase, Interacts with 26 S Protease Regulatory Subunit 4, Encoded by the PSMC1 Gene.

Czub B, Shah AZ, Alfano G, Kruczek PM, Chakarova CF, Bhattacharya SS

The significance of the ubiquitin-proteasome system (UPS) for protein degradation has been highlighted in the context of neurodegenerative diseases, including retinal dystrophies. TOPORS, a dual E3 ubiquitin and SUMO1 ligase, forms a component of the UPS and selected substrates for its enzymatic activities, such as DJ-1/PARK7 and APOBEC2, are important for neuronal as well as retinal homeostasis, respectively. TOPORS is ... [more]

PLoS ONE Feb. 13, 2016; 11(2);e0148678 [Pubmed: 26872363]

Throughput

  • Low Throughput

Curated By

  • BioGRID