VHL
Gene Ontology Biological Process
- NLS-bearing protein import into nucleus [IMP]
- angiogenesis [IMP]
- blood vessel endothelial cell migration [IMP]
- extracellular matrix organization [IMP]
- negative regulation of thymocyte apoptotic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription from RNA polymerase II promoter in response to hypoxia [ISO]
- neuron differentiation [ISO]
- positive regulation of transcription, DNA-templated [ISO]
- proteasomal protein catabolic process [IMP]
- protein catabolic process [IMP]
- protein heterooligomerization [ISO]
- protein transport [IDA]
- protein ubiquitination [ISO]
- regulation of apoptotic signaling pathway [IGI]
- regulation of catecholamine metabolic process [ISO]
- regulation of thymocyte apoptotic process [IGI]
- regulation of transcription, DNA-templated [ISO]
- response to hypoxia [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EGLN1
Gene Ontology Biological Process
- cardiac muscle tissue morphogenesis [IMP]
- heart trabecula formation [IMP]
- labyrinthine layer development [IMP]
- negative regulation of cAMP catabolic process [ISO]
- negative regulation of cyclic-nucleotide phosphodiesterase activity [ISO]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO]
- oxidation-reduction process [ISO]
- oxygen homeostasis [ISO]
- peptidyl-proline hydroxylation to 4-hydroxy-L-proline [ISO]
- regulation of angiogenesis [IMP]
- response to hypoxia [ISO]
- response to nitric oxide [ISO]
- ventricular septum morphogenesis [IMP]
Gene Ontology Molecular Function- enzyme binding [ISO]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [ISO]
- peptidyl-proline 4-dioxygenase activity [ISO]
- peptidyl-proline dioxygenase activity [ISO]
- enzyme binding [ISO]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [ISO]
- peptidyl-proline 4-dioxygenase activity [ISO]
- peptidyl-proline dioxygenase activity [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
RHOBTB3 promotes proteasomal degradation of HIFα through facilitating hydroxylation and suppresses the Warburg effect.
Hypoxia-inducible factors (HIFs) are master regulators of adaptive responses to low oxygen, and their α-subunits are rapidly degraded through the ubiquitination-dependent proteasomal pathway after hydroxylation. Aberrant accumulation or activation of HIFs is closely linked to many types of cancer. However, how hydroxylation of HIFα and its delivery to the ubiquitination machinery are regulated remains unclear. Here we show that Rho-related ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 3
Curated By
- BioGRID