SLX4
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [ISO]
- DNA double-strand break processing involved in repair via single-strand annealing [ISO]
- DNA repair [ISO]
- double-strand break repair via homologous recombination [ISO]
- nucleotide-excision repair [ISO]
- positive regulation of catalytic activity [ISO]
- response to intra-S DNA damage checkpoint signaling [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
USP45
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
USP45 deubiquitylase controls ERCC1-XPF endonuclease-mediated DNA damage responses.
Reversible protein ubiquitylation plays important roles in various processes including DNA repair. Here, we identify the deubiquitylase USP45 as a critical DNA repair regulator. USP45 associates with ERCC1, a subunit of the DNA repair endonuclease XPF-ERCC1, via a short acidic motif outside of the USP45 catalytic domain. Wild-type USP45, but not a USP45 mutant defective in ERCC1 binding, efficiently deubiquitylates ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 2
Curated By
- BioGRID