An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

USP45 deubiquitylase controls ERCC1-XPF endonuclease-mediated DNA damage responses.

Perez-Oliva AB, Lachaud C, Szyniarowski P, Munoz I, Macartney T, Hickson I, Rouse J, Alessi DR

Reversible protein ubiquitylation plays important roles in various processes including DNA repair. Here, we identify the deubiquitylase USP45 as a critical DNA repair regulator. USP45 associates with ERCC1, a subunit of the DNA repair endonuclease XPF-ERCC1, via a short acidic motif outside of the USP45 catalytic domain. Wild-type USP45, but not a USP45 mutant defective in ERCC1 binding, efficiently deubiquitylates ... [more]

EMBO J. Feb. 03, 2015; 34(3);326-43 [Pubmed: 25538220]

Throughput

Low Throughput

Additional Notes

Table S1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
USP45 MYH10	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Sowa ME (2009)	High	18.81	BioGRID	423174
MYH10 USP45	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Liu L (2023)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

USP45

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase activity [IBA]ubiquitin thiolesterase activity [IBA]ubiquitin-specific protease activity [IDA]

MYH10

Gene Ontology Biological Process

Gene Ontology Molecular Function

ADP binding [IDA]ATP binding [IDA, NAS]actin binding [NAS]actin filament binding [IDA, IMP]actin-dependent ATPase activity [IDA, IMP]microfilament motor activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS