BAIT
TLR3
AI957183
toll-like receptor 3
GO Process (32)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- I-kappaB phosphorylation [ISO]
- cellular response to exogenous dsRNA [ISO]
- defense response [IMP]
- defense response to virus [IGI, TAS]
- extrinsic apoptotic signaling pathway [ISO]
- innate immune response [TAS]
- microglial cell activation [ISO]
- microglial cell activation involved in immune response [ISO]
- necroptotic signaling pathway [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA, IGI, IMP]
- positive regulation of JNK cascade [IDA]
- positive regulation of NF-kappaB import into nucleus [ISO]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of chemokine biosynthetic process [IMP]
- positive regulation of chemokine production [ISO]
- positive regulation of interferon-alpha biosynthetic process [ISO]
- positive regulation of interferon-beta biosynthetic process [ISO, ISS]
- positive regulation of interferon-beta production [IDA]
- positive regulation of interferon-gamma biosynthetic process [ISO]
- positive regulation of interleukin-12 production [IDA]
- positive regulation of interleukin-6 production [IDA, IMP, ISO]
- positive regulation of interleukin-8 production [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of toll-like receptor signaling pathway [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of tumor necrosis factor production [IDA, IMP]
- positive regulation of type I interferon production [IDA]
- positive regulation of type III interferon production [IDA]
- response to dsRNA [ISO]
- response to exogenous dsRNA [IMP, ISO]
- response to virus [IMP]
- toll-like receptor signaling pathway [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
TRAF6
2310003F17Rik, AI851288, C630032O20Rik, RP23-313G3.1
TNF receptor-associated factor 6
GO Process (44)
GO Function (13)
GO Component (12)
Gene Ontology Biological Process
- I-kappaB kinase/NF-kappaB signaling [IMP]
- JNK cascade [ISO]
- T cell receptor signaling pathway [ISO]
- T-helper 1 type immune response [IMP]
- activation of NF-kappaB-inducing kinase activity [ISO]
- activation of protein kinase activity [ISO]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [IMP]
- bone remodeling [IMP]
- bone resorption [IMP]
- cell development [IMP]
- cellular response to lipopolysaccharide [ISO]
- cytokine-mediated signaling pathway [ISO]
- immune response [IMP]
- interleukin-1-mediated signaling pathway [IMP, ISO]
- myeloid dendritic cell differentiation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- neural tube closure [IMP]
- odontogenesis of dentin-containing tooth [IMP]
- organ morphogenesis [IMP]
- ossification [IMP]
- osteoclast differentiation [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IGI, IMP, ISO]
- positive regulation of JUN kinase activity [ISO]
- positive regulation of NF-kappaB transcription factor activity [IMP, ISO]
- positive regulation of T cell cytokine production [ISO]
- positive regulation of T cell proliferation [IMP]
- positive regulation of interleukin-12 biosynthetic process [IMP]
- positive regulation of interleukin-2 production [ISO]
- positive regulation of interleukin-6 biosynthetic process [IMP]
- positive regulation of lipopolysaccharide-mediated signaling pathway [IMP]
- positive regulation of osteoclast differentiation [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of smooth muscle cell proliferation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription regulatory region DNA binding [ISO]
- protein K63-linked ubiquitination [IDA, ISO]
- protein autoubiquitination [ISO, TAS]
- protein complex assembly [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IDA, IGI, IMP]
- regulation of immunoglobulin secretion [IDA]
- response to interleukin-1 [ISO]
- signal transduction [IDA, TAS]
Gene Ontology Molecular Function- histone deacetylase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase B binding [ISO]
- protein kinase binding [ISO]
- signal transducer activity [TAS]
- thioesterase binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, IMP]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
- histone deacetylase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase B binding [ISO]
- protein kinase binding [ISO]
- signal transducer activity [TAS]
- thioesterase binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, IMP]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Glycogen synthase kinase 3β ubiquitination by TRAF6 regulates TLR3-mediated pro-inflammatory cytokine production.
TRAF6 is critical for the production of inflammatory cytokines in various TLR-mediated signalling pathways. However, it is poorly understood how TRAF6 regulates TLR3 responses. Here we demonstrate that GSK3β interacts with TRAF6 and positively regulates the TLR3-mediated signalling. Suppression of GSK3β expression or its kinase activity drastically reduces the production of inflammatory cytokines and the induction of c-Fos by decreasing ... [more]
Nat Commun Apr. 02, 2015; 6(0);6765 [Pubmed: 25828701]
Throughput
- Low Throughput
Curated By
- BioGRID