An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.

Zlatic SA, Tornieri K, L'Hernault SW, Faundez V

Coats define the composition of carriers budding from organelles. In addition, coats interact with membrane tethers required for vesicular fusion. The yeast AP-3 (Adaptor Protein Complex 3) coat and the class C Vps/HOPS (HOmotypic fusion and Protein Sorting) tether follow this model as their interaction occurs at the carrier fusion step. Here we show that mammalian Vps class C/HOPS subunits ... [more]

Mol. Biol. Cell May. 15, 2011; 22(10);1699-715 [Pubmed: 21411634]

Throughput

Low Throughput

Additional Notes

Figure 1

Curated By

BioGRID

Interaction Summary

AP3D1

Gene Ontology Biological Process

Gene Ontology Molecular Function

transporter activity [TAS]

Gene Ontology Cellular Component

VPS18