BAIT
CHAF1A
AL023013, AL024058, CAF-1, Cac1p
chromatin assembly factor 1, subunit A (p150)
GO Process (3)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
TRP53BP1
53BP1, Tp53bp1, m53BP1, p53BP1, RP23-437L13.2
transformation related protein 53 binding protein 1
GO Process (5)
GO Function (9)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II transcription cofactor activity [ISO]
- damaged DNA binding [IDA]
- methylated histone binding [ISO]
- p53 binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- telomeric DNA binding [IDA]
- transcription factor binding [TAS]
- RNA polymerase II activating transcription factor binding [ISO]
- RNA polymerase II transcription cofactor activity [ISO]
- damaged DNA binding [IDA]
- methylated histone binding [ISO]
- p53 binding [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- telomeric DNA binding [IDA]
- transcription factor binding [TAS]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Systematic identification of factors for provirus silencing in embryonic stem cells.
Embryonic stem cells (ESCs) repress the expression of exogenous proviruses and endogenous retroviruses (ERVs). Here, we systematically dissected the cellular factors involved in provirus repression in embryonic carcinomas (ECs) and ESCs by a genome-wide siRNA screen. Histone chaperones (Chaf1a/b), sumoylation factors (Sumo2/Ube2i/Sae1/Uba2/Senp6), and chromatin modifiers (Trim28/Eset/Atf7ip) are key determinants that establish provirus silencing. RNA-seq analysis uncovered the roles of Chaf1a/b and ... [more]
Cell Sep. 24, 2015; 163(1);230-45 [Pubmed: 26365490]
Throughput
- High Throughput
Additional Notes
- Figure 7
Curated By
- BioGRID