FN1
Gene Ontology Biological Process
- blood coagulation [TAS]
- cell adhesion [NAS]
- endodermal cell differentiation [IDA]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- integrin activation [IMP]
- leukocyte migration [TAS]
- peptide cross-linking [IDA]
- platelet activation [TAS]
- platelet degranulation [TAS]
- response to wounding [NAS]
- substrate adhesion-dependent cell spreading [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ITGB1
Gene Ontology Biological Process
- B cell differentiation [IC]
- axon guidance [TAS]
- blood coagulation [TAS]
- calcium-independent cell-matrix adhesion [IGI]
- cell junction assembly [TAS]
- cell migration [TAS]
- cell-cell adhesion mediated by integrin [IEP]
- cell-matrix adhesion [IMP]
- cell-substrate adhesion [IMP]
- cellular defense response [TAS]
- extracellular matrix organization [TAS]
- heterotypic cell-cell adhesion [IMP]
- homophilic cell adhesion via plasma membrane adhesion molecules [TAS]
- integrin-mediated signaling pathway [IMP]
- leukocyte cell-cell adhesion [IDA]
- leukocyte migration [TAS]
- leukocyte tethering or rolling [IMP]
- mesodermal cell differentiation [IEP]
- negative regulation of anoikis [IMP]
- positive regulation of apoptotic process [IGI]
- positive regulation of establishment of protein localization to plasma membrane [IDA]
- regulation of collagen catabolic process [IDA]
- regulation of immune response [TAS]
- stress fiber assembly [IMP]
- transforming growth factor beta receptor signaling pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell surface [IDA]
- cytoplasm [IDA]
- extracellular vesicular exosome [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- integrin alpha1-beta1 complex [IDA]
- integrin alpha10-beta1 complex [IDA]
- integrin alpha11-beta1 complex [IDA]
- integrin alpha2-beta1 complex [IDA]
- integrin alpha3-beta1 complex [IDA]
- integrin alpha8-beta1 complex [TAS]
- integrin complex [NAS]
- invadopodium membrane [IDA]
- membrane [IDA]
- membrane raft [IDA]
- neuromuscular junction [IDA]
- plasma membrane [IDA, NAS, TAS]
- receptor complex [IDA]
- ruffle [TAS]
- ruffle membrane [IDA, NAS]
- sarcolemma [IDA]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration.
Membrane trafficking of integrins plays a pivotal role in cell proliferation and migration. How endocytosed integrins are targeted either for recycling or lysosomal delivery is not fully understood. Here, we show that fibronectin (FN) binding to α5β1 integrin triggers ubiquitination and internalization of the receptor complex. Acidification facilitates FN dissociation from integrin α5β1 in vitro and in early endosomes, promoting receptor ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FN1 ITGB1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 826606 | |
| FN1 ITGB1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 826028 | |
| FN1 ITGB1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3434752 | |
| FN1 ITGB1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| FN1 ITGB1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID