An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interplay of Endosomal pH and Ligand Occupancy in Integrin Î±5Î²1Â Ubiquitination, Endocytic Sorting, and Cell Migration.

Kharitidi D, Apaja PM, Manteghi S, Suzuki K, Malitskaya E, Roldan A, Gingras MC, Takagi J, Lukacs GL, Pause A

Membrane trafficking of integrins plays a pivotal role in cell proliferation and migration. How endocytosed integrins are targeted either for recycling or lysosomal delivery is not fully understood. Here, we show that fibronectin (FN) binding to Î±5Î²1 integrin triggers ubiquitination and internalization of the receptor complex. Acidification facilitates FN dissociation from integrin Î±5Î²1 inÂ vitro and in early endosomes, promoting receptor ... [more]

Cell Rep Oct. 20, 2015; 13(3);599-609 [Pubmed: 26456826]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

UBAP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ubiquitin binding [IDA]

Gene Ontology Cellular Component

ITGA5