An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A non-proteolytic role for ubiquitin in deadenylation of MHC-I mRNA by the RNA-binding E3-ligase MEX-3C.

Cano F, Rapiteanu R, Sebastiaan Winkler G, Lehner PJ

The regulation of protein and mRNA turnover is essential for many cellular processes. We recently showed that ubiquitin--traditionally linked to protein degradation--directly regulates the degradation of mRNAs through the action of a newly identified family of RNA-binding E3 ubiquitin ligases. How ubiquitin regulates mRNA decay remains unclear. Here, we identify a new role for ubiquitin in regulating deadenylation, the initial ... [more]

Nat Commun Oct. 17, 2015; 6(0);8670 [Pubmed: 26471122]

Throughput

High Throughput

Additional Notes

Table S1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MEX3C MYCBP2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Wolf EJ (2020)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MEX3C

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]

MYCBP2

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS