BAIT
RNF126
ring finger protein 126
GO Process (10)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
- cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process [IMP]
- negative regulation of epidermal growth factor receptor signaling pathway [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K48-linked ubiquitination [ISS]
- protein K63-linked ubiquitination [ISS]
- protein monoubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA, IMP]
- regulation of cell proliferation [IMP]
- retrograde transport, endosome to Golgi [IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
Gene Ontology Molecular Function
Homo sapiens
PREY
PFKP
ATP-PFK, PFK-C, PFK-P, PFKF, RP11-298E9.2
phosphofructokinase, platelet
GO Process (5)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Ubiquitin-Activated Interaction Traps (UBAITs) identify E3 ligase binding partners.
We describe a new class of reagents for identifying substrates, adaptors, and regulators of HECT and RING E3s. UBAITs (Ubiquitin-Activated Interaction Traps) are E3-ubiquitin fusion proteins and, in an E1- and E2-dependent manner, the C-terminal ubiquitin moiety forms an amide linkage to proteins that interact with the E3, enabling covalent co-purification of the E3 with partner proteins. We designed UBAITs ... [more]
EMBO Rep. Dec. 01, 2015; 16(12);1699-712 [Pubmed: 26508657]
Throughput
- Low Throughput
Additional Notes
- The UBAITs (Ubiquitin-Activated Interaction Traps) method was used.
Curated By
- BioGRID