BAIT

SHH

HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS, tcag7.582
sonic hedgehog
GO Process (70)
GO Function (6)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

EDEM3

C1orf22
ER degradation enhancer, mannosidase alpha-like 3
GO Process (4)
GO Function (0)
GO Component (1)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

EDEM2 and OS-9 Are Required for ER-Associated Degradation of Non-Glycosylated Sonic Hedgehog.

Tang HY, Huang CH, Zhuang YH, Christianson JC, Chen X

Misfolded proteins of the endoplasmic reticulum (ER) are eliminated by the ER-associated degradation (ERAD) in eukaryotes. In S. cerevisiae, ER-resident lectins mediate substrate recognition through bipartite signals consisting of an unfolded local structure and the adjacent glycan. Trimming of the glycan is essential for the directional delivery of the substrates. Whether a similar recognition and delivery mechanism exists in mammalian ... [more]

PLoS ONE Jun. 10, 2014; 9(6);e92164 [Pubmed: 24910992]

Throughput

  • Low Throughput

Curated By

  • BioGRID