An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Perturbation of the mutated EGFR interactome identifies vulnerabilities and resistance mechanisms.

Li J, Bennett K, Stukalov A, Fang B, Zhang G, Yoshida T, Okamoto I, Kim JY, Song L, Bai Y, Qian X, Rawal B, Schell M, Grebien F, Winter G, Rix U, Eschrich S, Colinge J, Koomen J, Superti-Furga G, Haura EB

We hypothesized that elucidating the interactome of epidermal growth factor receptor (EGFR) forms that are mutated in lung cancer, via global analysis of protein-protein interactions, phosphorylation, and systematically perturbing the ensuing network nodes, should offer a new, more systems-level perspective of the molecular etiology. Here, we describe an EGFR interactome of 263 proteins and offer a 14-protein core network critical ... [more]

Mol. Syst. Biol. Nov. 06, 2013; 9(0);705 [Pubmed: 24189400]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDC37 GFAP	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2709860

Curated By

BioGRID

Interaction Summary

CDC37

Gene Ontology Biological Process

Gene Ontology Molecular Function

chaperone binding [IBA]heat shock protein binding [IPI]kinase binding [IPI]protein binding [IPI]unfolded protein binding [TAS]

Gene Ontology Cellular Component

GFAP