An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A dual role of the N-terminal FQQI motif in GLUT4 trafficking.

Bernhardt U, Carlotti F, Hoeben RC, Joost HG, Al-Hasani H

In adipocytes, the glucose transporter GLUT4 recycles between intracellular storage vesicles and the plasma membrane. GLUT4 is internalized by a clathrin- and dynamin-dependent mechanism, and sorted into an insulin-sensitive storage compartment. Insulin stimulation leads to GLUT4 accumulation on the cell surface. The N-terminal F5QQI motif in GLUT4 has been shown previously to be required for sorting of the protein in ... [more]

Biol. Chem. Sep. 01, 2009; 390(9);883-92 [Pubmed: 19558319]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SLC2A4

Gene Ontology Biological Process

Gene Ontology Molecular Function

glucose transmembrane transporter activity [TAS]protein binding [IPI]

Gene Ontology Cellular Component

AP1M1