BAIT

POL1

CDC17, CRT5, HPR3, DNA-directed DNA polymerase alpha catalytic subunit POL1, L000000257, YNL102W
Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
Saccharomyces cerevisiae (S288c)
PREY

CTF18

CHL12, L000000431, L000000325, YMR078C
Subunit of a complex with Ctf8p; shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Genetic Networks Required to Coordinate Chromosome Replication by DNA Polymerases α, δ, and ε in Saccharomyces cerevisiae.

Dubarry M, Lawless C, Banks AP, Cockell S, Lydall D

Three major DNA polymerases replicate the linear eukaryotic chromosomes. DNA polymerase α-primase (Pol α) and DNA polymerase δ (Pol δ) replicate the lagging-strand and Pol α and DNA polymerase ε (Pol ε) the leading-strand. To identify factors affecting coordination of DNA replication, we have performed genome-wide quantitative fitness analyses of budding yeast cells containing defective polymerases. We combined temperature-sensitive mutations ... [more]

G3 (Bethesda) Oct. 01, 2015; 5(10);2187-97 [Pubmed: 26297725]

Throughput

  • High Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Additional Notes

  • Table S2
  • genome knock-out and DAmP collections used to create double mutants
  • pol1-4 allele
  • quantitative fitness analysis performed on double mutants constructed via SGA technique

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
POL1 CTF18
Dosage Lethality
Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Low-BioGRID
153813

Curated By

  • BioGRID