BAIT

HYP2

TIF51A, translation elongation factor eIF-5A, eIF-5A, eIF5A, L000000847, L000002307, YEL034W
Translation elongation factor eIF-5A; required for translation of proteins containing polyproline stretches, including Bni1p, and this leads to a requirement for mating projection formation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

SRP14

L000002061, YDL092W
Signal recognition particle (SRP) subunit; interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

eIF5A has a function in the cotranslational translocation of proteins into the ER.

Rossi D, Galvao FC, Bellato HM, Boldrin PE, Andrews BJ, Valentini SR, Zanelli CF

The putative eukaryotic translation initiation factor 5A (eIF5A) is a highly conserved and essential protein present in all organisms except bacteria. To be activated, eIF5A requires the conversion of a specific residue of lysine into hypusine. This hypusine modification occurs posttranslationally in two enzymatic steps, and the polyamine spermidine is the substrate. Despite having an essential function in translation elongation, ... [more]

Amino Acids Mar. 01, 2014; 46(3);645-53 [Pubmed: 24306454]

Throughput

  • Low Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Additional Notes

  • Figure 3C
  • SRP complex overexpression rescues temperature-sensitivity of tif51-A
  • genetic complex

Curated By

  • BioGRID