An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The variant Polycomb Repressor Complex 1 component PCGF1 interacts with a pluripotency sub-network that includes DPPA4, a regulator of embryogenesis.

Oliviero G, Munawar N, Watson A, Streubel G, Manning G, Bardwell V, Bracken AP, Cagney G

PCGF1 encodes one of six human Polycomb RING finger homologs that are linked to transcriptional repression and developmental gene regulation. Individual PCGF proteins define discrete Polycomb Repressor Complex 1 (PRC1) multi-protein complexes with diverse subunit composition whose functions are incompletely understood. PCGF1 is a component of a variant PRC1 complex that also contains the BCL6 co-repressor BCOR and the histone ... [more]

Sci Rep Dec. 22, 2015; 5(0);18388 [Pubmed: 26687479]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

PCGF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein C-terminus binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

CLIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

metal ion binding [NAS]microtubule binding [IDA]microtubule plus-end binding [IDA]protein binding [IPI]protein homodimerization activity [TAS]tubulin binding [IDA]zinc ion binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

The variant Polycomb Repressor Complex 1 component PCGF1 interacts with a pluripotency sub-network that includes DPPA4, a regulator of embryogenesis.

Throughput

Curated By

protein C-terminus binding [IPI]
protein binding [IPI]

metal ion binding [NAS]
microtubule binding [IDA]
microtubule plus-end binding [IDA]
protein binding [IPI]
protein homodimerization activity [TAS]
tubulin binding [IDA]
zinc ion binding [IDA]