BAIT

BRL2

BRI1-like 2, F14H20.2, F14H20_2, VASCULAR HIGHWAY 1, VH1, AT2G01950
serine/threonine-protein kinase BRI1-like 2
Arabidopsis thaliana (Columbia)
PREY

AT2G19940

F6F22.3, F6F22_3
probable N-acetyl-gamma-glutamyl-phosphate reductase
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

VH1/BRL2 receptor-like kinase interacts with vascular-specific adaptor proteins VIT and VIK to influence leaf venation.

Ceserani T, Trofka A, Gandotra N, Nelson T

VH1/BRL2 is a receptor-like kinase of the BRI1 family with a role in vascular development. In developing Arabidopsis leaves it is expressed first in ground cells and then becomes restricted to provascular and procambial cells as venation forms. We isolated proteins interacting with the activated (phosphorylated) cytoplasmic domain of VH1/BRL2, and found that most belong to three processes: proteasome activity, ... [more]

Plant J. Mar. 01, 2009; 57(6);1000-14 [Pubmed: 19000166]

Throughput

  • Low Throughput

Curated By

  • BioGRID