An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The BARD1 BRCT domain contributes to p53 binding, cytoplasmic and mitochondrial localization, and apoptotic function.

Tembe V, Martino-Echarri E, Marzec KA, Mok MT, Brodie KM, Mills K, Lei Y, DeFazio A, Rizos H, Kettle E, Boadle R, Henderson BR

BARD1 is a breast cancer tumor suppressor with multiple domains and functions. BARD1 comprises a tandem BRCT domain at the C-terminus, and this sequence has been reported to target BARD1 to distinct subcellular locations such as nuclear DNA breakage sites and the centrosome through binding to regulatory proteins such as HP1 and OLA1, respectively. We now identify the BRCT domain ... [more]

Cell. Signal. Sep. 01, 2015; 27(9);1763-71 [Pubmed: 26022179]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

BARD1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [IDA]kinase binding [NAS]protein binding [IPI]protein heterodimerization activity [IPI]protein homodimerization activity [IPI]ubiquitin-protein transferase activity [IDA, NAS]

Gene Ontology Cellular Component

HSPA4