STX17
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [IMP]
- Golgi organization [IMP]
- autophagic vacuole fusion [IMP]
- endoplasmic reticulum-Golgi intermediate compartment organization [IMP]
- intracellular protein transport [IBA]
- protein localization to pre-autophagosomal structure [IDA]
- vesicle docking [IBA]
- vesicle fusion [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ER to Golgi transport vesicle [ISS]
- ER-mitochondrion membrane contact site [IDA]
- SNARE complex [IDA]
- autophagic vacuole membrane [IDA]
- cytosol [ISS]
- endoplasmic reticulum membrane [IDA]
- endoplasmic reticulum-Golgi intermediate compartment [IDA]
- integral component of membrane [IBA]
- lysosomal membrane [IDA]
- mitochondrion [IDA]
- rough endoplasmic reticulum [IDA]
- smooth endoplasmic reticulum membrane [ISS]
VPS33A
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17.
Membrane fusion is generally controlled by Rabs, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), and tethering complexes. Syntaxin 17 (STX17) was recently identified as the autophagosomal SNARE required for autophagosome-lysosome fusion in mammals and Drosophila. In this study, to better understand the mechanism of autophagosome-lysosome fusion, we searched for STX17-interacting proteins. Immunoprecipitation and mass spectrometry analysis identified vacuolar protein sorting ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| STX17 VPS33A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID