Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

β-TrCP-mediated ubiquitination and degradation of liver-enriched transcription factor CREB-H.

Cheng Y, Gao WW, Tang HM, Deng JJ, Wong CM, Chan CP, Jin DY

CREB-H is an endoplasmic reticulum-resident bZIP transcription factor which critically regulates lipid homeostasis and gluconeogenesis in the liver. CREB-H is proteolytically activated by regulated intramembrane proteolysis to generate a C-terminally truncated form known as CREB-H-ΔTC, which translocates to the nucleus to activate target gene expression. CREB-H-ΔTC is a fast turnover protein but the mechanism governing its destruction was not well ... [more]

Sci Rep Apr. 01, 2016; 6(0);23938 [Pubmed: 27029215]

Throughput

  • Low Throughput

Curated By

  • BioGRID