Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Demonstration of the interaction of thioredoxin with p40phox, a phagocyte oxidase component, using a yeast two-hybrid system.

Nishiyama A, Ohno T, Iwata S, Matsui M, Hirota K, Masutani H, Nakamura H, Yodoi J

Thioredoxin (TRX) has disulfide reducing activity and is reported to be involved in various cellular functions including the promotion of cell growth and apoptosis. To help understand the molecular mechanism through which TRX is involved in immunological systems, we screened a cDNA library derived from a B-cell population of Epstein-Barr virus-transformed human peripheral blood lymhocyte for TRX binding proteins by ... [more]

Immunol. Lett. May. 03, 1999; 68(1);155-9 [Pubmed: 10397171]

Throughput

  • Low Throughput

Curated By

  • BioGRID