RAB13
Gene Ontology Biological Process
- GTP catabolic process [ISO]
- Rab protein signal transduction [IBA]
- cellular response to insulin stimulus [ISO]
- cortical actin cytoskeleton organization [IBA, ISO]
- endocytic recycling [IMP, ISO]
- endosomal transport [ISO]
- endothelial cell chemotaxis [IMP]
- establishment of Sertoli cell barrier [ISO]
- establishment of protein localization to plasma membrane [ISO]
- intracellular protein transport [TAS]
- neuron projection development [ISO]
- protein kinase A signaling [ISO]
- protein localization to cell leading edge [IMP]
- protein localization to plasma membrane [ISO]
- tight junction assembly [IBA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cell [IMP]
- cell-cell junction [IDA]
- cytoplasm [ISO]
- cytoplasmic membrane-bounded vesicle [TAS]
- cytoplasmic vesicle [ISO]
- endocytic vesicle [IDA, ISO]
- endosome [IBA]
- extracellular vesicular exosome [ISO]
- extrinsic component of plasma membrane [TAS]
- insulin-responsive compartment [ISO]
- intracellular [IMP]
- lamellipodium [IDA]
- lateral plasma membrane [ISO]
- neuron projection [ISO]
- plasma membrane [ISO]
- recycling endosome [ISO]
- tight junction [IDA, ISO]
- trans-Golgi network [ISO]
MICALL2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin.
The dynamic turnover of tight junctions (TJs) is essential for epithelial-mesenchymal transitions and/or mesenchymal-epithelial transitions during epithelial morphogenesis. We previously demonstrated that Rab13 specifically mediates the endocytic recycling of occludin. Here, we identified MICAL-L2 (molecule interacting with CasL-like 2) as a novel Rab13-binding protein. Immunoprecipitation and immunofluorescence microscopy showed that MICAL-L2 specifically bound to the GTP-bound form of Rab13 via ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RAB13 MICALL2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RAB13 MICALL2 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID